Studies on Madariaga virus (MADV), a potential emerging threat
MADV, an underexplored member of the eastern equine encephalitis virus complex, poses a health threat to humans and equines. In 2019, we isolated MADV from two horses that died of neurological disease in Northeast Brazil. We are currently conducting various studies to better understand the transmission cycle, patogenicity/virulence, and distribution of the virus, as well as assessing whether Texas mosquitoes could transmit the virus if it were introduced into the state.
Region in Brazil where we isolated MADV in 2019 (Fig. published in Gil et al. Pathogens, 2021).
Exploring epidemiological aspects of Zika, dengue and chikungunya viruses within human cohorts
We have been conducting research in Northeast Brazil with human cohorts to study various epidemiological aspects of Zika, dengue, and chikungunya viruses. Our research has involved detecting ongoing outbreaks, exploring the role of sexual transmission in Zika epidemiology, and assessing humoral immunity profiles against arboviruses over time. These studies aim to help us better understand the spatiotemporal variation in arboviral disease epidemiology.
Hotspots of Zika virus and chikungunya virus infections during outbreaks in a region of Northeast Brazil in 2015-2016 (Fig. published in Magalhaes et al. Plos Negl Trop Dis 2017).
The relevance of sexual transmission of Zika virus was assessed through a retrospective cohort study conducted in households in Northeast Brazil (Magalhaes et al. J Infect Dis, 2021).
Field simulations: mosquito biology and arbovirus transmission
From a mosquito biology standpoint, various scenarios can occur in the field when arboviruses are circulating within a geographic area. For instance, female mosquitoes can ingest different arboviruses from different hosts, as well as antibodies and even medicine taken by humans. In the laboratory, we simulate these scenarios to assess virus transmission outcomes. This data can contribute to our understanding of disease epidemiology and may be used for modeling studies on arboviral disease transmission.
The biology of mosquito vectors must be considered in the epidemiology and transmission patterns or arboviruses. In our laboratory, we simulate various field scenarios and incorporate mosquito biology features in the experiments to evaluate arbovirus transmission outcomes.
Magalhaes T, Portilho MM, Moreira PSS, Marinho ML, Dias WP, Gonçalves NM, Rodrigues OAS, Montes J, Reis L, Jesus DF, Silva TO, Dultra LS, Nery JS, Ribeiro GS. Validation of the use of dried blood spots in a chikungunya virus IgG serological assay. J Immunol Methods (accepted for publication).
Rosenberger KD, Khanh LP, Tobian F, et al. Early diagnostic indicators of dengue versus other febrile illnesses in Asia and Latin America (IDAMS study): a multicentre, prospective, observational study. Lancet Global Health 2023; 11(3):e361-72.
Foy BD, Magalhaes T, Some A, Gray L, Rao S, Sougue E, Jackson CL, Kittleson J, Slater HC, Bousema T, Ollo D, Coulidiaty G, Colt M, Wade M, Richards K, Fabrice Some A, Dabire RK, Parikh S. Repeat Ivermectin Mass Drug Administrations for Malaria Control II: a double-blind, cluster-randomized control trial for integrated control of malaria. Protocol for the RIMDAMAL II Trial. JMIR Research Protocols 2023; 12:1-13.
DOI : 10.2196/41197
Magalhaes T, Morais CNL, Azevedo EAN, Jacques IJAA, Castanha PMS, Cordeiro MT, Braga C, Jaenisch T, Marques ETA, Foy BD. Two-year decay of Zika virus neutralizing antibodies in people living in an endemic region in Brazil. Am J Trop Med Hyg 2022; 107(1):186-189.
Cardoso CW, Souza MMS, Teles ACVS, Argibay HD, Reis OB, Pereira FM, Giovanetti M, Magalhaes T, Ribeiro GS. COVID-19 outbreaks among crew members in non-cruise vessels anchoring in Salvador, Brazil, 2021. Mem Inst Oswaldo Cruz 2022; 117:1-8.
Gil L*, Magalhaes T*, Santos B, et al. Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil. Pathogens 2021; 10. *Equal contribution
Magalhaes T, Morais CNL, Jacques I, et al. Follow-Up Household Serosurvey in Northeast Brazil for Zika Virus: Sexual Contacts of Index Patients Have the Highest Risk for Seropositivity. J Infect Dis 2021; 223:673-85.
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Chotiwan N, Brewster CD, Magalhaes T, et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Sci Transl Med 2017; 9.
Bosch I, de Puig H, Hiley M, et al. Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci Transl Med 2017; 9.
Kassar TC, Magalhaes T, Junior SJ, et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. An Acad Bras Cienc 2017; 89:2119-30.
Freire M, Pol-Fachin L, Coelho DF, et al. Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses. ACS Omega2017; 2:3913-20.